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February 24, 2016 by Kevin Keras

Let's Go Cycling!

Thermal Cycler Verification

Occasionally, your PCR yields may not be what you expected or have been experiencing.   A great deal of time can be spent looking at primers and buffers, or even your DNA samples but as is often the case...you find out it is a thermal cycler issue.   It usually happens at just the wrong time too...

Adding a temperature verification of your thermal cycler block (or blocks) is a great way to beef up your SOP's to ensure that your PCR runs smoothly.

Here at TheLabSquad we run a 12 point (probe) test as part on our standard PM procedure. It only takes about 15 minutes per block but you wind up with a graph of the ramps and a PASS/FAIL score.  This is done using an ISO 17025 certified tool that is calibrated annually.

As with any process, ensuring that your major variables are accounted for upfront makes a huge difference in your end results.

So, if you haven't had your thermal cycler  PM'd in awhile, now might be a good time to check under the hood (lid) before you go cycling.


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